S. Nishii et al., Determination of bisphenol A by ELISA using an organic solvent-resistant anti-bisphenol A monoclonal antibody, BUNSEKI KAG, 49(12), 2000, pp. 969-975
Bisphenol A(BPA), which is used as a starting material of epoxy resins and
polycarbonates, is suspected as being one of the endocrine-disrupting chemi
cals. Generally, BPA analysis requires an expensive instrument, such as GC/
MS, and time-consuming processes, such as extraction, concentration, and de
rivatization. We have developed a rapid, simple, and highly sensitive analy
tical method of BPA by enzyme-linked immnosorbent assay(ELISA) using an org
anic solvent-resistant anti-BPA monoclonal antibody in order to solve such
problems. In order to produce anti-BPA monoclonal antibodies, Hybridoma cel
ls were produced by the fusion of mouse myeloma cells and spleen cells from
mice immunized with BPA-bovine serum albumin conjugates (PA-BSA). Some ant
i-BPA monoclonal antibodies were found to have resistance to methanol. They
selectively react with BPA, but do not show a cross-reaction with structur
ally related compounds. The detection limit of BPA by ELISA using the organ
ic solvent resistant antibody was 0.5 ppb or less, and the detection limit
in 50% methanol was 1 ppb. The measurement of BPA is possible even in other
organic solvents, such as ethanol, acetone,DMSO and acetonitrile, if the o
rganic-solvent concentration in the sample is 30% or less. This ELISA syste
m provides an analytical method which does not affect environment for detec
ting die BPA concentration in river water and blood specimens. In this pape
r, various analysis examples using the ELISA method are introduced.