Fabrication of a miniaturized cell based on electrochemiluminescence of Ru(bpy)(3)(2+) and its application to the determination of some peptides

Authors
Egashira, N Piao, J Hifumi, E Uda, T
Citation
N. Egashira et al., Fabrication of a miniaturized cell based on electrochemiluminescence of Ru(bpy)(3)(2+) and its application to the determination of some peptides, BUNSEKI KAG, 49(12), 2000, pp. 1029-1031
Citations number
10
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
BUNSEKI KAGAKU
ISSN journal
05251931 → ACNP
Volume
49
Issue
12
Year of publication
2000
Pages
1029 - 1031
Database
ISI
SICI code
0525-1931(200012)49:12<1029:FOAMCB>2.0.ZU;2-5
Abstract
A miniaturized cell based on electrochemiluminescence of tris(2,2'-bipyridi ne) ruthenium (II) complex was prepared to determine peptides in small volu me of 0.5 cm(3) A calibration for proline gave a straight line in the conce ntration range 1.0 x 10(-5)similar to1.0 x 10(-3) M (correlation coefficien t: 0.991). Stronger responses for proline and histidine than other amino ac ids prompted us to measure some peptides Out of six peptides, PG having pro line at the amino terminal showed the strongest response.