Recurrent chromosome changes in 62 primary gastric carcinomas detected by comparative genomic hybridization

Comparative genomic hybridization (CGH) has been applied to detect recurren t chromosome alterations in 62 primary gastric carcinomas. Several nonrando m chromosomal changes, including gains of 8q (31 cases, 50%), 20q (29 cases , 47%) with a minimum gain region at 20q11.2-q12, 13q (21 cases, 34%) with a minimum gain region at 13q22, and 3q (19 cases, 31%) were commonly observ ed. The regions most frequently lost included: 19p (23 cases, 37%), 37p (21 cases, 33%), and Ip (14 cases, 23%). High copy number gain (DNA sequence a mplification) was detected in 6 cases. Amplification of 8q23-q24.2 and 20q1 1.2-q12 were observed in 3 cases. Gain of 20q and loss of 19p were confirme d by fluorescence in situ hybridization using corresponding bacterial artif icial chromosomes (BAC) clones from those regions. The gain and loss of chr omosomal regions identified in this study provide candidate regions involve d in gastric tumorigenesis. (C) 2000 Elsevier Science Inc. All rights reser ved.