Current concepts of fat graft survival: Histology of aspirated adipose tissue and review of the literature

BACKGROUND. Controversy remains about the longevity of correction in autolo gous fat grafts and its relation to adipocyte survival. Reported long-term fat graft survival rates differ widely, depending on harvesting method, mea ns of reinjection, injection site, and evaluation methods. OBJECTIVE. To demonstrate histologic findings of aspirated adipose tissue a nd compare the findings to the reports in the literature. METHODS. Review of the literature and the histology of transplanted fat 7 y ears after subcutaneous implantation and trypan blue staining to determine the vitality of defrosted adipocytes. RESULTS. Fat cells survive aspiration with a suction machine or syringe equ ally well. Use of a liposuction cannula or 14-gauge needle gives comparable results. Local anesthesia or tumescent local anesthesia is recommended for the donor site, preferably with addition of epinephrine. CONCLUSION. Clinical longevity of correction after autologous fat transfer is determined by the degree of augmentation resulting from the amount of fi brosis induced and the number of viable fat cells. Survival of aspirated fa t cell grafts depends mainly on the anatomic site, the mobility and vascula rity of the recipient tissue, or underlying causes and diseases, and less o n harvesting and reinjection methods.