HIGH PRODUCTION AND USE OF ROUGH ANTIGENS FOR SEROLOGICAL DIAGNOSIS OF GAMBIAN TRYPANOSOMIASIS BY ELISA AND LATEX AGGLUTINATION TECHNIQUES

The extraction of rough antigens (Ag) from trypanosomes came accross a major problem getting of a high number of parasites, Moreover several technics for the extraction of rough antigens are described. Some of them are sophisticated and time consuming. Aiming the obtention of a l arge number of Ag for this study inoculation of trypanosomes into Cric etomys gambianus and affinity chromatography of blood allow to collect of large number of trypanosomes. This new technique leads to the extr action of 50 to 60 mg of rough Ag of trypanosomes isolated from infect ed Cricetomys. In order to set up a specific and sensitive serological diagnosis of gambian human trypanosomiasis, the different rough Ag we re used comparatively by ELISA and Latex Agglutination tests. Confirmi ng previous works, the results indicate id high sensitivity and a doub tful specificity for both techniques.