Baboon cytochrome P450c11 is encoded by more than one gene

Authors
Swart, AC Brown, N Kolar, N Swart, P
Citation
Ac. Swart et al., Baboon cytochrome P450c11 is encoded by more than one gene, ENDOCRINE R, 26(4), 2000, pp. 1011-1018
Citations number
5
Categorie Soggetti
Endocrinology, Nutrition & Metabolism
Journal title
ENDOCRINE RESEARCH
ISSN journal
07435800 → ACNP
Volume
26
Issue
4
Year of publication
2000
Pages
1011 - 1018
Database
ISI
SICI code
0743-5800(2000)26:4<1011:BCPIEB>2.0.ZU;2-G
Abstract
Two P450c11 genes, sharing 99% homology, were cloned from Cape baboon adren al tissue using RT-PCR. The cDNAs showed 96% and 94.6% sequence identity to human P450c11 and aldosterone synthase, respectively. One of the gene sequ ences contained a termination codon in exon one. The cloned cDNAs were expr essed in COS 1 cells and the metabolism of deoxycorticosterone to corticost erone investigated. The expressed enzyme exhibited 11beta hydroxylase activ ity, but no aldosterone synthase activity.