Allelic selection of human IL-2 gene

Citation
F. Matesanz et al., Allelic selection of human IL-2 gene, EUR J IMMUN, 30(12), 2000, pp. 3516-3521
Citations number
26
Categorie Soggetti
Immunology
Journal title
EUROPEAN JOURNAL OF IMMUNOLOGY
ISSN journal
00142980 → ACNP
Volume
30
Issue
12
Year of publication
2000
Pages
3516 - 3521
Database
ISI
SICI code
0014-2980(200012)30:12<3516:ASOHIG>2.0.ZU;2-O
Abstract
The allelic expression of mouse IL-2 cannot be definitely extrapolated to w hat might happen in humans. Therefore, we investigated the regulation of al lelic expression of the IL-2 gene in non-genetically manipulated human T ly mphocytes by following natural allelic polymorphisms. We found a phenotypic ally silent punctual change in the human IL-2 at position 114 after the fir st nucleotide of the initiation codon, which represents a dimorphic polymor phism at the first exon of the IL-2 gene. This allowed the study by single- cell PCR of the regulation of the human IL-2 allelic expression in heterozy gous CD4(+) T cells, which was found to be tightly controlled monoallelical ly. These findings may be used as a suitable marker for monitoring the IL-2 allelic contribution to effector activities and in immune responses agains t different infections or in pathological situations.