Adenosine A(1) receptor blockade reverses dysmotility induced by ischemia-reperfusion in rat colon

This study was designed to assess whether adenosine A(1) receptor antagonis ts [(R)-1-[(E)-3-(2-phenylpyrazolo[1,5-a]pyridin-3-yl) acryloyl]-piperidin- 2-yl acetic acid (FK352) and 8-cyclopentyl-1,3-dipropylxanthine (DPCPX)] re verse dysmotility induced by ischemia-reperfusion in the rat colon. The gen e of adenosine A(1) receptor was expressed in the colon. Clamping (30 min) of the colonic marginal vessels was followed by reperfusion, and the propul sive colonic motility was evaluated. Propulsion was significantly slowed by ischemia-reperfusion, while FK352 and DPCPX abolished this delay. In contr ast, the non-selective adenosine receptor antagonist, 8-phenyltheophylline, failed to affect the dysmotility. Thus, adenosine A(1) receptor antagonist s have potent therapeutic potential against ischemia-reperfusion-induced dy smotility in the colon. (C) 2000 Elsevier Science B.V. All rights reserved.