Archaeal promoter-directed expression of the Halobacterium salinarum catalase-peroxidase gene

The Halobacterium salinarum catalase-peroxidase gene was subcloned into shu ttle vectors pWL102 and pWL202 and expressed under the control of different archaeal promoters. When Hbr. salinarum was transformed with the catalase- peroxidase gene under the control of its own promoter, catalase-peroxidase activity increased twofold. Catalase-peroxidase activity increased threefol d when Hbt. salinarum was transformed with the catalase-peroxidase gene und er the control of a tRNA promoter. This bifunctional enzyme in Hbt. salinar um was not induced by environmental stresses such as H2O2, intense light, d arkness, high temperature, low temperature, redox inhibitors, heavy metals, or ions.