Role of the Gp160 from HIV-1 m7NDK CD4 independent isolate in CXCR4 down-regulation

Authors
Valente, S Chanel, C Dumonceaux, J Olivier, R Marullo, S Briand, P Hazan, U
Citation
S. Valente et al., Role of the Gp160 from HIV-1 m7NDK CD4 independent isolate in CXCR4 down-regulation, FIFTH EUROPEAN CONFERENCE ON EXPERIMENTAL AIDS RESEARCH (ECEAR 2000), 2000, pp. 127-133
Citations number
6
Categorie Soggetti
Current Book Contents
Journal title
FIFTH EUROPEAN CONFERENCE ON EXPERIMENTAL AIDS RESEARCH (ECEAR 2000) → ACNP
Year of publication
2000
Pages
127 - 133
Database
ISI
SICI code
Abstract
We have previously characterized the first CD4-independent HIV-I X4 isolate m7NDK and showed a direct byding of this mutant gp120 with CXCR4. We prese nt evidence of down-regulation of CXCR4 membrane expression in CD4 positive cells chronically infected with the HIV-I m7NDK. This was shown by FAGS an alysis and the phenomenon was associated with a drastic reduction of steady state mRNA level. Kinetic analysis showed that CXCR4 is down-regulated in CEM cells within a maximum of six days post HIV-I m7NDK infection. Expressi on of the envelope protein might be responsible for this negative modulatio n of CXCR4.