The full-length 5' and 3' untranslated regions (UTRs) of Xenopus laevis Con
nexin30 (Cx30) mRNA were cloned and sequenced. The Cx30 messenger contains
a 148 nt 5' UTR and a 480 nt 3' UTR. Four different constructs were made to
enable the analysis of the role of the Cx30 UTRs in translation efficiency
and in protein localization in the early Xenopus embryo. Transcripts encod
ed the Green Fluorescent Protein (GFP) reporter and contained the 5' and 3'
UTR of either Cx30 or globin. In vivo analyses after injection of the tran
scripts into one cell-stage Xenopus embryos showed that the Cx30 3' UTR ena
bles very efficient translation. The 5' UTR was slightly inhibitory compare
d with the globin 5' UTR. The localization of the produced GFP was analyzed
. GFP was ubiquitously expressed in all parts of the embryo. Based on this
observation we conclude that neither the 5' UTR nor the 3' UTR confers spec
ific localization of the translation of the Cx30 mRNA in the embryo. (C) 20
00 Elsevier Science B.V. All rights reserved.