Group A streptococcal rofA gene is involved in the control of several virulence genes and eukaryotic cell attachment and internalization

The serotype M6 group A streptococcal RofA regulator was previously shown t o exert a direct positive control of protein F1 expression and, concomitant ly, fibronectin binding. Using a serotype M6 rofA mutant, we demonstrate he re that this regulator has a potentially indirect negative influence on the expression of the mga, emm6 pel-sagA, and speA virulence genes. Additional ly, the rofA mutant exhibited reduced eukaryotic cell internalization rates in combination with decreased host cell viability.