Expression of ExsA in trans confers type III secretion system-dependent cytotoxicity on noncytotoxic Pseudomonas aeruginosa cystic fibrosis isolates

Authors
Dacheux, D Attree, I Toussaint, B
Citation
D. Dacheux et al., Expression of ExsA in trans confers type III secretion system-dependent cytotoxicity on noncytotoxic Pseudomonas aeruginosa cystic fibrosis isolates, INFEC IMMUN, 69(1), 2001, pp. 538-542
Citations number
29
Categorie Soggetti
Immunology
Journal title
INFECTION AND IMMUNITY
ISSN journal
00199567 → ACNP
Volume
69
Issue
1
Year of publication
2001
Pages
538 - 542
Database
ISI
SICI code
0019-9567(200101)69:1<538:EOEITC>2.0.ZU;2-#
Abstract
Twelve Pseudomonas aeruginosa cystic fibrosis isolates that are not able to exert a type III secretion system (TTSS)-dependent cytotoxicity towards ph agocytes have been further studied. The strains, although possessing TTSS g enes and exsA, which encodes a positive regulator of the TTSS regulon, show ed no transcriptional activation of the exsCBA regulatory operon. The expre ssion of exsA in trans restored the in vitro secretion of TTSS proteins and ex vivo cytotoxicity.