VACCINATION WITH HIV-1 GP120 DNA INDUCES IMMUNE-RESPONSES THAT ARE BOOSTED BY A RECOMBINANT GP120 PROTEIN SUBUNIT

Citation
Sw. Barnett et al., VACCINATION WITH HIV-1 GP120 DNA INDUCES IMMUNE-RESPONSES THAT ARE BOOSTED BY A RECOMBINANT GP120 PROTEIN SUBUNIT, Vaccine, 15(8), 1997, pp. 869-873
Citations number
10
Categorie Soggetti
Immunology
Journal title
ISSN journal
0264410X
Volume
15
Issue
8
Year of publication
1997
Pages
869 - 873
Database
ISI
SICI code
0264-410X(1997)15:8<869:VWHGDI>2.0.ZU;2-Y
Abstract
Small animals were immunized with plasmid DNA encoding HIV-1 envelope gp120 either intramuscularly by needle injection (mice and guinea pigs ) or epidermally with the Accell(TM) gene gun (guinea pigs). Subsequen tly, the animals were boosted with a recombinant gp120 protein subunit vaccine in an oil-in-water based adjuvant, MF59. Antibodies and cytot oxic T-lymphocyte (CTL) immune responses to the HIV envelope glycoprot ein were observed in animals immunized with gp120 DNA derived from the HIV-1(SF2), laboratory strain or from HIV-1 field isolates. Titers of ELISA antibodies and serum neutralizing antibodies against the HIV-1( SF2) laboratory isolate were substantially increased in DNA-immunized animals following a single boost with recombinant gp120 protein subuni t. This DNA prime/protein subunit boost immunization approach may be i mportant for vaccination against infectious agents such as HIV for whi ch it is difficult to raise strong antiviral humoral responses with DN A vaccination alone. (C) 1997 Elsevier Science Ltd.