The functional ratio of chymase and angiotensin converting enzyme in angiotensin I-induced vascular contraction in monkeys, dogs and rats

Recently, a chymase-dependent angiotensin (Ang)II-forming pathway was found in human cardiovascular tissues, and the significance of this pathway in t he pathogenesis of some cardiovascular diseases was suggested. The present study examined the ratio of angiotensin converting enzyme (ACE) to chymase- dependent Ang II formation in various isolated vessels from monkeys, dogs a nd rats. In all of the examined vessels, the addition of KCl at a concentra tion of 50 mM could induce a maximal contraction. Except for monkey coronar y artery and rat renal and femoral artery, the addition of Ang I could indu ce transitory contractions, whereas the force of contractions in these vess els was quite different. The sensitivity to Ang II in these vessels was sim ilar to that for Ang I. In monkey gastroepiploic and mesenteric arteries, a bout 70% of the Ang I-induced contraction was suppressed by chymase inhibit ion, while it was suppressed about 50% in monkey renal, femoral and carotid arteries. In dog renal arteries, about 65% of the Ang I-induced contractio n was suppressed by chymase inhibition, while it was suppressed by about 30 % in other dog arteries. In contrast, in all rat arteries, AngI-induced con tractions were completely suppressed by treatment with ACE inhibitor alone. We concluded that regional differences in the response to Ang I exist in v ascular tissues, and the ratio of ACE- to chymase-dependent Ang II formatio n is different in the various vessels.