Mb. Avison et al., A TEM-2 beta-lactamase encoded on an active Tn1-like transposon in the genome of a clinical isolate of Stenotrophomonas maltophilia, J ANTIMICRO, 46(6), 2000, pp. 879-884
A constitutively expressed beta -lactamase gene from a clinical isolate of
Stenotrophomonas maltophilia, J675Ia, has been cloned. Its DNA sequence is
almost identical to that of bla(TEM2) (one nucleotide change) and the expre
ssed enzyme is a Bush type 2a penicillinase with an amino acid sequence ide
ntical to that of TEM-2, The bla(TEM) gene was present within a novel Tn1/T
n3-type transposon in the genome of isolate J675Ia and the transposon was a
ble to mobilize bla(TEM) on to the broad host-range conjugative plasmid, R3
88. When transferred to an Escherichia coli recipient, R388::Tn conferred h
igh-level ampicillin resistance. This represents the first identification o
f a TEM beta -lactamase in S. maltophilia and the first evidence that this
important clinical pathogen is able to act as a reservoir for mobile beta -
lactamase genes in the hospital environment.