Application of monoliths for downstream processing of clotting factor IX

In this paper, the application of monolithic columns for downstream process ing of different clotting factor IX concentrates is shown. Determination of basic chromatographic conditions as well as investigations on the regenera tion of disk- and tube-shaped monolithic columns using human serum albumin as a model protein, were performed. Separation of factor IX and vitronectin , a possible impurity in commercial factor IX concentrates was accomplished using disk-shaped monolithic columns. These same applications were also ca rried out with identical results on up-scaled tube-shaped monolithic column s. Since these media allow very fast separations, this method can be succes sfully applied not only to an in-process control of the purification of fac tor IX but also to other biopolymers from human plasma. Besides, the same a pplication on the up-scaled tube-shaped monolithic column was successfully carried out. (C) 2000 Elsevier Science B.V. All rights reserved.