Chromatographic study of terpene derivatives on porous graphitic carbon stationary phase with beta-cyclodextrin as mobile phase modifier

The stoichiometric coefficients and apparent formation constants (K-f) of a lpha -terpineol, thymol, geraniol and linalool complexes with beta -cyclode xtrin (beta -CD) were determined using HPLC with a porous graphitic carbon (PGC) chromatographic support. Measurements were performed with four differ ent methanol-water mobile phases. All the terpene derivatives under study f orm 1:1 guest-CD complexes. Graphs of K-f as a function of the mobile phase composition appeared different from those classically described for RP-C-1 8 and suggest that the PGC stationary phase could play an active role in th e complexation process. Solute-CD inclusion and solute-stationary phase int eractions may be involved in this specific behavior. (C) 2000 Elsevier Scie nce B.V. All rights reserved.