Planar chromatography with electroosmotic flow is used to separate either a
mixture of dyes using 80% aqueous ethanol as the mobile phase or a mixture
of miscellaneous compounds using 45% aqueous acetonitrile as the mobile ph
ase. Both mobile phases are 1.0 mM in N-[tris(hydroxymethyl)methyl]-3-amino
propanesulfonic acid (TAPS) buffer. Separations using this technique are fa
ster and more efficient than the same separations by conventional TLC. The
respective relationships between migration velocity and applied potential,
and between analysis time and distance migrated, are presented. (C) 2000 El
sevier Science B.V. All rights reserved.