Separation and determination of haloperidol, parabens and some of their degradation products by micellar electrokinetic chromatography

Citation
R. Driouich et al., Separation and determination of haloperidol, parabens and some of their degradation products by micellar electrokinetic chromatography, J CHROMAT A, 903(1-2), 2000, pp. 271-278
Citations number
21
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
Volume
903
Issue
1-2
Year of publication
2000
Pages
271 - 278
Database
ISI
SICI code
Abstract
A micellar solution containing phosphate buffer, anionic surfactant, and wa ter-miscible organic solvent was employed as a migration solution for the s eparation and the quantification of eleven analytes by micellar electrokine tic chromatography (MEKC): the analytes examined were haloperidol, methylpa raben, ethylparaben, n-propylparaben, iso-propylparaben, n-butylparaben, is o-butylparaben, sec-butylparaben, 4-(4-chlorophenyl)-4-hydroxypiperidine, 4 -fluorobenzoic acid and 4-hydroxybenzoic acid. In order to provide good sep aration between micelle and haloperidol, which showed strongest interaction with the micelle among the analytes, surfactant concentrations and organic modifier percentages were studied with phosphate buffer at pH 7.0. All the analytes were successfully resolved when 10 mM sodium dodecylsulfate and 1 5% ethanol were contained in the migration solution; the time window was ve ry wide in the range from 14.8 to 65.5 min. Optimized applied voltage at 30 kV and capillary temperature at 45 degreesC enable analyze all compounds i n less than 17 min with the best resolution, the shorter migration time win dow, the highest precision and lowest detection limit. (C) 2000 Elsevier Sc ience B.V. All rights reserved.