The induction of cell death in human osteoarthritis chondrocytes by nitricoxide is related to the production of prostaglandin E-2 via the induction of cyclooxygenase-2

There is increasing evidence suggesting that chondrocyte death may contribu te to the progression of osteoarthritis (OA), This study focused on the cha racterization of signaling cascade during NO-induced cell death in human OA chondrocytes, The NO generator, sodium nitroprusside (SNP), promoted chond rocyte death in association with DNA fragmentation, caspase-3 activation, a nd down-regulation of Bcl-2, Both caspase-3 inhibitor Z-Asp(OCH3)-Glu(OCH3) -Val-Asp(OCH3)-CH2F and caspase-9 inhibitor Z-Leu-Glu(OCH3)-His-Asp(OCH3)-C H2F prevented the chondrocyte death. Blocking the mitogen-activated protein kinase pathway by the mitogen-activated protein kinase kinase 1/2 inhibito r PD98059 or p38 kinase inhibitor SB202190 also inhibited the SNP-mediated cell death, suggesting possible requirements of both extracellular signal-r elated protein kinase 1/2 and p38 kinase for the NO-induced cell death. Fur thermore, the selective inhibition of cyclooxygenase (COX)-2 by NS-398 or t he inhibition of COX-1/COX-2 by indomethacin blocked the SNP-induced cell d eath. The chondrocyte death induced by SNP was associated with an overexpre ssion of COX-2 protein las determined by Western blotting) and an increase in PGE, release, PD98059 and SB202190, but neither Z-DEVD FMK nor Z-LEHD FM K completely inhibited the SNP-mediated PGE, production, Analysis of intera ctions between PGE, and the cell death showed that PGE, enhanced the SNP-me diated cell death, whereas PGE, alone did not induce the chondrocyte death. These data indicate that NO-induced chondrocyte death signaling includes P GE, production via COX-2 induction and suggest that both extracellular sign al-related protein kinase 1/2 and p38 kinase pathways are upstream signalin g of the PGE, production. The results also demonstrate that exogenous PGE, may sensitize human OA chondrocytes to the cell death induced by NO.