Preconception immunization with a cytomegalovirus (CMV) glycoprotein vaccine improves pregnancy outcome in a guinea pig model of congenital CMV infection

Authors
Bourne, N Schleiss, MR Bravo, FJ Bernstein, DI
Citation
N. Bourne et al., Preconception immunization with a cytomegalovirus (CMV) glycoprotein vaccine improves pregnancy outcome in a guinea pig model of congenital CMV infection, J INFEC DIS, 183(1), 2001, pp. 59-64
Citations number
29
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Immunology
Journal title
JOURNAL OF INFECTIOUS DISEASES
ISSN journal
00221899 → ACNP
Volume
183
Issue
1
Year of publication
2001
Pages
59 - 64
Database
ISI
SICI code
0022-1899(20010101)183:1<59:PIWAC(>2.0.ZU;2-K
Abstract
The guinea pig (gp) model of congenital cytomegalovirus (CMV) infection was used to evaluate a gpCMV glycoprotein vaccine. Hartley guinea pigs were im munized 3 times with 50 mug of lectin column-purified glycoproteins prepare d from gpCMV-infected or -uninfected tissue culture. Immunization with the gpCMV vaccine produced seroconversion in all animals. Animals then were pla ced with gpCMV-seronegative male animals and were challenged late in pregna ncy with virulent salivary gland-passaged gpCMV. Immunization with gpCMV gl ycoproteins significantly improved pregnancy outcome, with 54 of 63 pups li ve-born in immunized animals, compared with 21 of 48 in the controls (P<.00 1). In addition, virus was isolated from 24 of 54 live-born pups born to im munized mothers, compared with 16 of 20 live-born pups born to controls, in dicating that immunization significantly reduced in utero transmission in s urviving animals (P<.01).