A single amino acid substitution in the ICP27 protein of herpes simplex virus type 1 is responsible for its resistance to leptomycin B

Authors
Murata, T Goshima, F Koshizuka, T Takakuwa, H Nishiyama, Y
Citation
T. Murata et al., A single amino acid substitution in the ICP27 protein of herpes simplex virus type 1 is responsible for its resistance to leptomycin B, J VIROLOGY, 75(2), 2001, pp. 1039-1043
Citations number
40
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022538X → ACNP
Volume
75
Issue
2
Year of publication
2001
Pages
1039 - 1043
Database
ISI
SICI code
0022-538X(200101)75:2<1039:ASAASI>2.0.ZU;2-V
Abstract
Leptomycin B (LMB) is a specific inhibitor of Crm1-dependent nuclear export of proteins. The replication of herpes simplex virus (HSV) is normally hig hly sensitive to LMB; a resistant HSV variant, however, was isolated by ser ial passages of the virus. Analysis of marker transfer and viral DNA sequen ces revealed that a single amino acid substitution within the ICP27 gene is responsible for conferring this resistance.