Genomic bacterial artificial chromosome library of the Japanese flounder Paralichthys olivaceus

Authors
Katagiri, T Asakawa, S Hirono, I Aoki, T Shimizu, N
Citation
T. Katagiri et al., Genomic bacterial artificial chromosome library of the Japanese flounder Paralichthys olivaceus, MAR BIOTEC, 2(6), 2000, pp. 571-576
Citations number
11
Categorie Soggetti
Aquatic Sciences
Journal title
MARINE BIOTECHNOLOGY
ISSN journal
14362228 → ACNP
Volume
2
Issue
6
Year of publication
2000
Pages
571 - 576
Database
ISI
SICI code
1436-2228(200011/12)2:6<571:GBACLO>2.0.ZU;2-0
Abstract
We have constructed a genomic bacterial artificial chromosome (BAC) library from homozygous cloned Japanese flounder Paralichthys olivaceus using the pBAC-lac vector. This BAC library consists of about 49,100 clones and is de posited in 128 microtiter plates with 384 wens. The average size of inserte d DNA was calculated to be 165 kb. The BAC library was determined to cover 9 times the Japanese founder haploid genome. The Japanese flounder genomic EPIC library will be useful for gene isolation as well as quantitative trai t loci (QTL) analysis.