A simple and rapid method for purifying staphylococcal exfoliative toxin A

Authors
Yamazaki, T Sakurai, S Matsuo, N Machida, K Kohno, M
Citation
T. Yamazaki et al., A simple and rapid method for purifying staphylococcal exfoliative toxin A, MICROB IMMU, 44(12), 2000, pp. 1067-1070
Citations number
15
Categorie Soggetti
Microbiology
Journal title
MICROBIOLOGY AND IMMUNOLOGY
ISSN journal
03855600 → ACNP
Volume
44
Issue
12
Year of publication
2000
Pages
1067 - 1070
Database
ISI
SICI code
0385-5600(2000)44:12<1067:ASARMF>2.0.ZU;2-1
Abstract
A rapid and efficient method of purification of staphylococcal exfoliative toxin A, the causative agent of staphylococcal scalded skin syndrome (SSSS) , has been developed. It is based on ammonium sulfate precipitation of the culture supernatant and hydrophobic interaction chromatography on Phenyl-Se pharose CL-4B. This procedure results in 87-fold purification of this toxin , which appears as a single band in sodium dodecyl sulfate-polyacrylamide g els.