Protective effects of hemin and tetrakis(4-benzoic acid)porphyrin on bacterial mutagenesis and mouse skin carcinogenesis induced by 7,12-dimethylbenz[a]anthracene
Yw. Chung et al., Protective effects of hemin and tetrakis(4-benzoic acid)porphyrin on bacterial mutagenesis and mouse skin carcinogenesis induced by 7,12-dimethylbenz[a]anthracene, MUT RES-GTE, 472(1-2), 2000, pp. 139-145
Citations number
41
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
Porphyrins which are widespread in nature can interfere with the actions of
certain carcinogens and mutagens, and have also been used clinically in ph
otodynamic therapy (PDT) of tumors. Porphyrins such as chlorophyll, chlorop
hyllin (CHL) and hemin are known to inactivate various mutagens by forming
complexes with them. Tetrakis(4-benzoic acid)porphyrin (TBAP) has been deve
loped as a photosensitizer for PDT and its metal complex, MnTBAP has been s
hown to be efficacious in a variety of in vitro and in vivo oxidative stres
s models of human diseases. In the present study, we have found that TBAP a
nd hemin exert concentration-related inhibition of his(+) reversion in Salm
onella typhimurium TA100 induced by 7,12-dimethylbenz[a]anthracene (DMBA),
and significantly reduced both incidence and multiplicity of skin tumors wh
en topically applied prior to treatment of 12-O-tetradecanoylphorbol-13-ace
tate in female ICR mice. Covalent DNA binding of DMBA in mouse skin was als
o significantly inhibited by topical application of TBAP or hemin as well a
s CHL. These results suggest the chemopreventive potential of compounds con
taining a porphyrin nucleus. (C) 2000 Elsevier Science B.V. All rights rese
rved.