The genetic toxicity of 3,3 ',4,4 '-tetrachloroazobenzene and 3,3 ',4,4 '-tetrachloroazoxybenzene: discordance between acute mouse bone marrow and subchronic mouse peripheral blood micronucleus test results
Kl. Witt et al., The genetic toxicity of 3,3 ',4,4 '-tetrachloroazobenzene and 3,3 ',4,4 '-tetrachloroazoxybenzene: discordance between acute mouse bone marrow and subchronic mouse peripheral blood micronucleus test results, MUT RES-GTE, 472(1-2), 2000, pp. 147-154
Citations number
33
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
3,3',4,4'-Tetrachloroazobenzene (TCAB) and 3,3',4,4'-tetrachloroazoxybenzen
e (TCAOB) are dioxin-like chemicals that were investigated for toxicity in
13-week gavage studies in male and female B6C3F(1) mice and F344N rats by t
he National Toxicology Program. As part of the comprehensive toxicological
investigation of these chemicals, peripheral blood smears from mice treated
5 days per week for 13 weeks with 0.1-30 mg/kg/day TCAB or TCAOB were anal
yzed for the frequency of micronucleated (MN) normochromatic erythrocytes (
NCE). Both chemicals produced significant increases in MN-NCE in male and f
emale mice. In contrast to these positive results in subchronic exposure st
udies, no significant increases were seen in acute bone marrow MN tests in
male mice administered three daily injections of 50-200 mg/kg/day TCAB and
TCAOB. The results with TCAB and TCAOB suggest that the routine integration
of MN rests with subchronic toxicity studies may allow detection of mutage
nic activity for some chemicals that fail to elicit responses in short-term
, high dose tests. In addition, the integration of mutagenicity tests into
general toxicity tests reduces the use of laboratory animals and the cost o
f the testing. (C) 2000 Published by Elsevier Science B.V.