The epitope recognized by the monoclonal antibody NM11, previously sho
wn to recognize both CBP and p300, has been mapped here to the C-termi
nal third of p300 and CBP by Western analysis of p300 and CBP prokaryo
tic fusion proteins, More precise epitope mapping, carried out by scre
ening a plasmid expression library derived from small randomly generat
ed CBP cDNA fragments localizes the NM11 epitope to a 21 amino acid st
retch spanning amino acids 2071-2091 near the CBP C-terminus. CBP and
p300 differ by three noncontiguous residues within this 21 amino acid
region, a difference that does not detectably affect the reactivity of
NM11.