Isolation and purification of the reaction center (RC) and the core (RC-LH1) complex from Rhodobium marinum: the LH1 ring of the detergent-solubilized core complex contains 32 bacteriochlorophylls

Authors
Qian, P Yagura, T Koyama, Y Cogdell, RJ
Citation
P. Qian et al., Isolation and purification of the reaction center (RC) and the core (RC-LH1) complex from Rhodobium marinum: the LH1 ring of the detergent-solubilized core complex contains 32 bacteriochlorophylls, PLANT CEL P, 41(12), 2000, pp. 1347-1353
Citations number
28
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT AND CELL PHYSIOLOGY
ISSN journal
00320781 → ACNP
Volume
41
Issue
12
Year of publication
2000
Pages
1347 - 1353
Database
ISI
SICI code
0032-0781(200012)41:12<1347:IAPOTR>2.0.ZU;2-9
Abstract
The reaction center (RC) and the core (RC-LH1) complex were isolated and pu rified from Rhodobium marinum; together with the LH1 complex [Meckenstock e t al. (1992a) FEES Lett, 311: 128], a complete set of RC, LH1 and RC-LH1 fr om the same wild-type strain of a purple photosynthetic bacterium can there fore now be made. Comparison of the BChl a/BPhe a ratio (determined by HPLC ) between the RC and the RC-LH1 complexes lead us to the determination of t he number of BChls in the LH1 ring to be 32.06 +/- 2.90, indicating that th e LH1 ring from Rh. marinum consists of 16 alpha beta subunits.