An enhancer trap line associated with a D-class cyclin gene in arabidopsis

Citation
K. Swaminathan et al., An enhancer trap line associated with a D-class cyclin gene in arabidopsis, PLANT PHYSL, 124(4), 2000, pp. 1658-1667
Citations number
23
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT PHYSIOLOGY
ISSN journal
00320889 → ACNP
Volume
124
Issue
4
Year of publication
2000
Pages
1658 - 1667
Database
ISI
SICI code
0032-0889(200012)124:4<1658:AETLAW>2.0.ZU;2-O
Abstract
In yeast and animals, cyclins have been demonstrated to be important regula tors of cell, cycle progression. In recent years, a large number of A-, B-, and D-class cyclins have been isolated from a variety of plant species. On e class of cyclins, the D-class cyclins, is important for progression throu gh G1 phase of the cell cycle. In Arabidopsis, four D-class cyclins have be en isolated and characterized (CYCLIN-D1;1, CYCLIN-D2;1, CYCLIN-D3;1, and C YCLIN-D4;1). In this report we describe the characterization of a fifth D-c lass cyclin gene, CYCLIN-D3;2 (CYCD3;2), from Arabidopsis. An enhancer trap line, Line 5580, contains a T-DNA insertion in CYCD3;2. Enhancer trap line 5580 exhibits expression in young vegetative and floral primordia. In Line 5580, T-DNA is inserted in the first exon of the CYCD3;2 gene; in homozygo us 5580 plants CYCD3;2 RNA is not detectable. Even though CYCD3;2 gene func tion is eliminated, homozygous 5580 plants do not exhibit an obvious growth or developmental phenotype. Via in situ hybridization we demonstrate that CYCD3;2 RNA is expressed in developing vegetative and floral primordia. In addition, CYCD3;2 is also capable of rescuing a yeast strain that is defici ent in G1 cyclin activity.