J. Wiedenmann et al., Cracks in the beta-can: Fluorescent proteins from Anemonia sulcata (Anthozoa, Actinaria), P NAS US, 97(26), 2000, pp. 14091-14096
Citations number
46
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
We characterize two green fluorescent proteins (GFPs), an orange fluorescen
t protein, and a nonfluorescent red protein isolated from the sea anemone A
nemonia sulcata. The orange fluorescent protein and the red protein seem to
represent two different states of the same protein. Furthermore. we descri
be the cloning of a GFP and a nonfluorescent red protein. Both proteins are
homologous to the GFP from Aequorea victoria. The red protein is significa
ntly smaller than other GFP homologues, and the formation of a closed GFP-l
ike beta -can is not possible. Nevertheless, the primary structure of the r
ed protein carries all features necessary for orange fluorescence. We discu
ss a type of beta -can that could be formed in a multimerization process.