A homology-based structure prediction method ideally gives both a correct f
old assignment and an accurate query-template alignment. In this article we
show that the combination of two existing methods, PSI-BLAST and threading
, leads to significant enhancement in the success rate of fold recognition.
The combined approach, termed COBLATH, also yields much higher alignment a
ccuracy than found in previous studies. It consists of two-way searches bot
h by PSI-BLAST and by threading. In the PSI-BLAST portion, a query is used
to search for hits in a library of potential templates and, conversely, eac
h potential template is used to search for hits in a library of queries. In
the threading portion, the scoring function is the sum of a sequence profi
le and a 6x6 substitution matrix between predicted query and known template
secondary structure and solvent exposure. "Two-way" in threading means tha
t the query's sequence profile is used to match the sequences of all potent
ial templates and the sequence profiles of all potential templates are used
to match the query's sequence. When tested on a set of 533 nonhomologous p
roteins, COBLATH was able to assign folds for 390 (73%). Among these 390 qu
eries, 265 (68%) had root-mean-square deviations (RMSDs) of less than 8 Ang
strom between predicted and actual structures. Such high success rate and a
ccuracy make COBLATH an ideal tool for structural genomics, Proteins 2001;4
2:23-37. (C) 2000 Wiley-Liss, Inc.