Nuclease protection of RNAs containing site-specific labels: A rapid method for mapping RNA-protein interactions

Authors
Maroney, PA Romfo, CM Nilsen, TW
Citation
Pa. Maroney et al., Nuclease protection of RNAs containing site-specific labels: A rapid method for mapping RNA-protein interactions, RNA, 6(12), 2000, pp. 1905-1909
Citations number
12
Categorie Soggetti
Biochemistry & Biophysics
Journal title
RNA-A PUBLICATION OF THE RNA SOCIETY
ISSN journal
13558382 → ACNP
Volume
6
Issue
12
Year of publication
2000
Pages
1905 - 1909
Database
ISI
SICI code
1355-8382(200012)6:12<1905:NPORCS>2.0.ZU;2-1
Abstract
This report describes a method that combines nuclease protection and site-s pecific labeling to determine sites and extents of RNA-protein interactions . The utility of the method is demonstrated by the analysis of the binding of factors to the 3' splice site region of a pre-mRNA labeled at three spec ific positions. This "reverse footprinting" technique should be widely appl icable to a variety of questions concerning RNA-protein interactions.