Design and applications of rapid-scan spectrally resolved fluorescence microscopy

This paper addresses the design and applications of a spectrally resolved c onfocal fluorescence microscope that measures spectral information for each pixel in the image with a fast multi-point approach. The combined spatial and spectral resolution of the device is demonstrated by performing two-pho ton imaging of mixtures of fluorescently labeled microspheres. Overlapping emission spectra of dye solutions contained in microcapillaries are readily distinguished. Images of a human skin tissue section and a mouse kidney se ction are presented which demonstrate that the structure and spectra of bio logic samples can be resolved.