A family of compact plasmid vectors for DNA immunization in humans

DNA immunization technology is based on the availability of adequate vector s for cloning and expression of heterologous immunoactive proteins in mamma lian cells. We have developed a family of DNA plasmid vectors suitable to m anipulate antigen expression and location. Their in vitro and in vivo funct ionality and application are also reported. The developed immune response, the aspects considered for vector design, and the possible independent mani pulation of both blocks for the generation of bicistronic constructs, make of the pAEC family of plasmid vectors a source for DNA vaccine candidate's development for further evaluation in human clinical trials, and for potent ial use in the gene therapy approach. (C) 2000 Academic Press.