Effects of overexpression of the liver subunit of 6-phosphofructo-1-kinaseon the metabolism of a cultured mammalian cell line

Overexpression of the liver subunit of 6-phosphofructo-1-kinase in Chinese hamster ovary K1 cells was shown to increase the steady-state level of the enzyme's product, fructose 1,6-bisphosphate, and to produce a small but sig nificant decrease in the concentration of fructose 2,6-bisphosphate, which is an allosteric activator of the enzyme. However, overexpression of the en zyme had no effect on glycolytic flux under a variety of different substrat e conditions. This latter observation is consistent with similar studies in fungi and in potato tubers which indicate that 6-phosphofructo-1-kinase ha s very little control over flux in glycolysis.