Cloning and preliminary characterization of a 105 kDa protein with an N-terminal kinase-like domain

In the course of searching for proteins that interact with protein kinase B in 3T3-L1 adipocytes, we isolated a 105 kDa protein from 3T3-L1 adipocytes . Peptides sequenced from the protein were found to be present in several e xpressed sequence tags. A cDNA containing one of these expressed sequence t ags was sequenced and appears to contain the entire coding region. Computer analysis revealed a potential protein kinase domain at the N-terminus; how ever, the first subdomain and several invariant residues characteristic of protein kinases are absent. An antibody was raised against a peptide from t he 105 kDa protein. By immunoblotting, it was found that the protein was wi dely expressed in mouse tissues, and concentrated in the cytosol and low de nsity microsome fractions of 3T3-L1 adipocytes. (C) 2000 Elsevier Science B .V. All rights reserved.