Sch. Liu et al., Cloning and preliminary characterization of a 105 kDa protein with an N-terminal kinase-like domain, BBA-GENE ST, 1517(1), 2000, pp. 148-152
Citations number
13
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
In the course of searching for proteins that interact with protein kinase B
in 3T3-L1 adipocytes, we isolated a 105 kDa protein from 3T3-L1 adipocytes
. Peptides sequenced from the protein were found to be present in several e
xpressed sequence tags. A cDNA containing one of these expressed sequence t
ags was sequenced and appears to contain the entire coding region. Computer
analysis revealed a potential protein kinase domain at the N-terminus; how
ever, the first subdomain and several invariant residues characteristic of
protein kinases are absent. An antibody was raised against a peptide from t
he 105 kDa protein. By immunoblotting, it was found that the protein was wi
dely expressed in mouse tissues, and concentrated in the cytosol and low de
nsity microsome fractions of 3T3-L1 adipocytes. (C) 2000 Elsevier Science B
.V. All rights reserved.