I. Shureiqi et al., 15-Lipoxygenase-1 mediates nonsteroidal anti-inflammatory drug-induced apoptosis independently of cyclooxygenase-2 in colon cancer cells, CANCER RES, 60(24), 2000, pp. 6846-6850
We previously found (I. Shureiqi et al., Carcinogenesis (Lond.), 20: 1985-1
995, 1999; I. Shureiqi et al., J. Natl. Cancer Inst., 92: 1136-1142, 2000)
that (a) 15-lipoxygenase-1 (15-LOX-1) protein and its product 13-S- hydroxy
octadecadienoic acid (13-S-HODE) are decreased; and (b) nonsteroidal anti-i
nflammatory drug (NSAID)-induced 15-LOX-1 expression is critical to NSAID-i
nduced apoptosis in colorectal cancer cells expressing cyclooxygenase-2 (CO
X-2). We used the NSAIDs sulindac sulfone (COX-2-independent) and NS-398 (a
COX-2 inhibitor) to assess NSAID upregulation of 15-LOX-1 in relation to C
OX-2 inhibition during NSAID-induced apoptosis in the DLD-1 (COX-2-negative
) colon cancer cell line. We found thats (a) NSAIDs up-regulated 15-LOX-1,
which preceded apoptosis; and (b) 15-LOX-1 inhibition blocked NSAID-induced
apoptosis, which was restored by 13-S-HODE but not by its parent, linoleic
acid. NSAIDs can induce apoptosis in colon cancer cells via up-regulation
of 15-LOX-1 in the absence of COX-2.