Spectral karyotyping of mouse cell line WMP2

We have evaluated the mouse cell line WMP2 using both GTG-banding analysis and spectral karyotyping to verify the reliability of using this establishe d cell line derived from WMP/WMP mice. The WMP cell lines contain easily id entifiable metacentric fusion chromosomes and are used extensively for gene mapping. Because of karyotypical changes in the WMP1 cell line, WMP2 was e xamined. Our results demonstrate that WMP2 is stable during culture, and th e karyotype is simple and easy to use. Based on the findings discussed in t his paper, we recommend the use of the WMP2 cell line for future prospectiv e gene mapping in the mouse. Copyright (C) 2000 S. Karger AG, Basel.