Study of biodistribution of methotrexate-loaded bovine serum albumin nanospheres in mice

Nanospheres made from natural hydrophilic polymers have been proved efficie nt in terms of better drug-loading capacity, biocompatibility, and possibil ity less opsonization by reticuloendothelial system (RES) through nn aqueou s stearic barrier Hence, nanospheres containing methotrexate were prepared from bovine serum albumin (BSA) by a novel pH coacervation method A drug-to -polymer ratio study was carried out to determine the carrier capacity. The batch with the highest drug lending was subjected to in vitro analysis. It was found to provide a slow release after an initial burst release. Biodis tribution of nanosphere-bound drug was compared with that of free drug in m ice. It was observed that the percentage increase in drug distribution to t he lungs, liver, and spleen was markedly high from the nanosphere when comp ared to free drug.