The isoprostanes: Their quantification as an index of oxidant stress status in vivo

The F-2 isoprostanes are a unique series of prostaglandinlike compounds for med in vivo from the fine-radical-catalyzed peroxidation of arachidonic aci d independent of the cyclooxygenase enzyme. The purpose of this review is t o briefly summarize the status of our current knowledge regarding the isopr ostanes. Novel aspects related to the biochemistry of isoprostane formation and methods by which these compounds are analyzed are discussed. The utili ty of measuring F-2 isoprostanes as markers of lipid peroxidation in animal models of oxidant stress are then outlined. Finally, examples are provided in which quantification of isoprostanes have illuminated the role of oxida tive injury in several human diseases.