Immobilization of lactate dehydrogenase on electrochemically prepared polypyrrole-polyvinylsulphonate composite films for application to lactate biosensors

Authors
Chaubey, A Gerard, M Singhal, R Singh, VS Malhotra, BD
Citation
A. Chaubey et al., Immobilization of lactate dehydrogenase on electrochemically prepared polypyrrole-polyvinylsulphonate composite films for application to lactate biosensors, ELECTR ACT, 46(5), 2000, pp. 723-729
Citations number
31
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
ELECTROCHIMICA ACTA
ISSN journal
00134686 → ACNP
Volume
46
Issue
5
Year of publication
2000
Pages
723 - 729
Database
ISI
SICI code
0013-4686(200012)46:5<723:IOLDOE>2.0.ZU;2-2
Abstract
The immobilization of lactate dehydrogenase (LDH) on electrochemically poly merized polypyrrole - polyvinylsulphonate (PPY-PVS) films has been accompli shed via cross-linking technique using glutaraldehyde. The characterization of the LDH-immobilized PPY-PVS films has been carried out using FTIR and c yclic voltammetry. These PPY-PVS-LDH electrodes are shown to have a detecti on limit of 1 x 10(-4) M, a response time of about 40 s, and a shelf-life o f about 2 weeks and these can be used for L-lactate estimation from 0.5 to 6 mM. (C) 2000 Elsevier Science Ltd. All rights reserved.