SCREENING FOR SALMONELLA WITH A MURINE MONOCLONAL-ANTIBODY M105 DETECTS BOTH FELIX O1 BACTERIOPHAGE SENSITIVE AND RESISTANT SALMONELLA STRAINS

Ten Felix O1 (FO1) bacteriophage sensitive Salmonella strains as well as their phage resistant derivates together with 39 strains of FO1-res istant Salmonella were tested for their reactivities with a murine mon oclonal antibody, M105, by indirect whole cell and competitive ELISA. All FO1 phage sensitive and 48 of the 49 FO1-resistant Salmonella stra ins were found to react with M105. The single Salmonella strain not re acting with M105 was a F01 resistant derivative selected by exposing t he sensitive parent strain to the phage. This M105-negative and FO1-re sistant strain was also found to be a rough mutant without O-antigens and possibly lacks the terminal LPS core sugars which form the M105 re active epitope.