Electrical stimulation accelerates and increases expression of BDNF and trkB rnRNA in regenerating rat femoral motoneurons

Electrical stimulation promotes the speed and accuracy of motor axonal rege neration. The positive effects of stimulation are mediated at the cell body . Here we characterize the effect of electrical stimulation on motoneuronal expression of BDNF and its receptor, trkB, two genes whose expression leve ls in motoneurons correlate with regeneration and are regulated by electric al activity in a variety of neurons. We used semiquantitative in situ hybri dization to measure expression of mRNA encoding BDNF and the full-length tr kB receptor at intervals of 8 h, 2 days and 7 days after unilateral femoral nerve cut, suture, and stimulation. Expression in regenerating motoneurons was compared to that of contralateral intact motoneurons. BDNF and trkB si gnals were not significantly upregulated 8 h and 2 days after femoral nerve suture and sham stimulation. By 7 days, there was a 2-fold increase in bot h BDNF and trkB mRNA expression. In contrast, stimulation of cut and repair ed nerves for only 1 h led to rapid upregulation of BDNF and trkB mRNA by 3 -fold and 2-fold, respectively, within the first 8 h. The stimulation effec t peaked at 2 days with 6-fold and 4-fold increases in the signals, respect ively. Thereafter, the levels of BDNF and trkB mRNA expression declined to equal the 2-fold increase seen at 7 days after nerve repair and sham-stimul ation. We conclude that brief electrical stimulation stimulates BDNF and tr kB expression in regenerating motoneurons. Because electrical stimulation i s known to accelerate axonal regeneration, we suggest that changes in the e xpression of BDNF and trkB correlate with acceleration of axonal regenerati on.