T-cell receptor repertoire expression in workers with occupational asthma due to platinum salt

Citation
M. Raulf-heimsoth et al., T-cell receptor repertoire expression in workers with occupational asthma due to platinum salt, EUR RESP J, 16(5), 2000, pp. 871-878
Citations number
31
Categorie Soggetti
Cardiovascular & Respiratory Systems","da verificare
Journal title
EUROPEAN RESPIRATORY JOURNAL
ISSN journal
09031936 → ACNP
Volume
16
Issue
5
Year of publication
2000
Pages
871 - 878
Database
ISI
SICI code
0903-1936(200011)16:5<871:TRREIW>2.0.ZU;2-T
Abstract
There is a high incidence of asthma, rhinitis, conjunctivitis and contact u rticaria in workers of precious metal refineries. Symptoms are closely asso ciated,vith sensitization to halogenated platinum compounds, as assessed by skin-prick test. The aim of the present study was to examine the molecular mechanisms involved by describing the T-cell receptor (TCR) repertoire dis tribution of peripheral blood mononuclear cells (PBMCs) without and after i n vitro stimulation with sodium hexachloroplatinate. PBMCs of 17 sensitized subjects,vith work-related asthma and a positive ski n-prick test result to sodium hexachloroplatinate and of 15 nonexposed subj ects were isolated and TCR expression determined by flow cytometry. Further more, the sodium hexachloroplatinate-mediated in vitro effects on the frequ ency of V beta -expressing T-cells, the proliferation response and the expr ession of cell surface molecules like CD71, CD25, CD95 and HLA-DR were stud ied. CD3-positive lymphocytes of platinum salt-sensitized workers had a signific antly higher frequency of V alpha 2a+, V beta 11+ and V beta 21.3+ T-cells than controls (p<0.01, p<0.01 and p<0.001 respectively), III vitro stimulat ion of PBMCs from platinum salt-sensitized as well as control subjects with sodium hexachloroplatinate increased the percentage of CD3-positive cells bearing specific TCRs, especially V<beta>5.3, V beta6.7, V beta 8a, V beta 20 and V beta 21.3. This effect was time- and dose-dependent. The present results indicate that the frequencies of V alpha 2a-, V beta 11 and V beta 21.3-bearing blood T-cells and platinum salt-induced lymphocyte proliferation are strongly enhanced in subjects who suffer from asthma due to platinum salt. In addition, in vitro stimulation with sodium hexachloro platinate modulates the frequencies of certain T-cell receptor-bearing T-ce lls.