Queen mating frequency is correlated with mature colony size across monogyn
ous ant species as a whole. However, recent comparisons between Atta and Ac
romyrmex leafcutter ants found an opposite trend. We tested whether this re
sult could be reproduced for Atta sexdens, using a new. Atta colombica micr
osatellite marker to analyse queens and worker offspring from six young col
onies. We found that all queens had mated multiply and used sperm from at l
east 2-3 males. This result is comparable to previous findings for. Atta co
lombica and seems to confirm that queen mating frequency in Atta is lower t
han in Acromyrmex.