Pseudomonas frederiksbergensis sp nov., isolated from soil at a coal gasification site

Phenotypic and genotypic characterization indicated that a group of 29 clos ely related phenanthrene-degrading bacteria from a coal gasification site i n Frederiksberg, Copenhagen, Denmark, belonged to the genus Pseudomonas. Th e strains were isolated at two sampling occasions 2 years apart. The isolat es were phenotypically different from any known species of the genus Pseudo monas and were therefore subject to further identification. Colonies were s mooth and pale yellowish and did not produce pigments fluorescent in UV lig ht when grown on King's B agar. Cells were rod-shaped, approximately 0.5-0. 8 x 1.5-3.0 mum, and grew at 4 and 30 degreesC, but not 37 degreesC. The ba cteria were oxidase- and catalase-positive, accumulated poly-beta -hydroxyb utyrate and denitrified, but did not utilize D-xylose. The mean G+C content was 59.6 mol%. Phenotypic data and 16S rDNA sequence data information for Pseudomonas amygdali and Pseudomonas corrugate, and 16S rDNA sequence data for Pseudomonas chlororaphis and Pseudomonas syringae showed close relation ships to these strains. However, DNA-DNA hybridization data showed that the isolates belong to a new species, for which the name Pseudomonas frederiks bergensis sp. nov, is proposed. The type strain is JAJ28(T) (DSM 13022(T)).