E. Hintermann et al., Expression of the melanin-concentrating hormone receptor in porcine and human ciliary epithelial cells, INV OPHTH V, 42(1), 2001, pp. 206-209
PURPOSE. To evaluate whether the receptors for melanin-concentrating hormon
e (MCH) and its functional antagonist alpha -melanocyte-stimulating hormone
(alpha -MSH) are expressed in the ciliary epithelium. Furthermore, to exam
ine whether MCH, a neuropeptide involved in fluid and electrolyte homeostas
is, may influence ion flux mediated by Na,K (adenosine triphosphatase)-ATPa
se in a ciliary epithelial cell line.
METHODS. Expression of MCH receptors (MCH-R) and alpha -MSH receptors (MSH-
R) on primary porcine ciliary pigmented epithelial (PE) cells and on a huma
n nonpigmented ciliary epithelial (NPE) cell line, ODM-2 was investigated b
y radioligand binding studies and reverse transcription-polymerase chain re
action (RT-PCR). The MCH-R nas further characterized by photocrosslinking.
Influence of MCH on Na,K;-ATPase activity Nas evaluated by an Rb+ transport
assay.
RESULTS. MCH-R expression was observed at both the mRNA and protein levels
in PE and NPE cells. In contrast, MSH-Rs were not detectable. At the mRNA l
evel, expression of slc-1 was shown and with crosslinking, a 44-kDa protein
was labeled. MCH showed no effect on Na,K-ATPase activity of NPE cells.
CONCLUSIONS. The presence of MCH-R in ciliary epithelial cells of both huma
n and porcine origin but the absence of MSPI-Rs indicates that in these cel
ls, MCH and alpha -MSH do not form a functionally antagonistic hormonal pai
r as they do in several other systems. Although effects of MCH on intestina
l water and ion transport have been documented, a direct control of Na,K-AT
Pase activity was not detected in human NPE cells in vitro.