SYNTHESIS OF THE DODECASACCHARIDE FRAGMENT REPRESENTING THE O-POLYSACCHARIDE OF VIBRIO-CHOLERAE 0 1, SEROTYPE OGAWA, BEARING AN AGLYCON OFFERING FLEXIBILITY FOR CHEMICAL LINKING TO PROTEINS/

Authors
Citation
Y. Ogawa et P. Kovac, SYNTHESIS OF THE DODECASACCHARIDE FRAGMENT REPRESENTING THE O-POLYSACCHARIDE OF VIBRIO-CHOLERAE 0 1, SEROTYPE OGAWA, BEARING AN AGLYCON OFFERING FLEXIBILITY FOR CHEMICAL LINKING TO PROTEINS/, Glycoconjugate journal, 14(4), 1997, pp. 433-438
Citations number
23
Categorie Soggetti
Biology
Journal title
ISSN journal
02820080
Volume
14
Issue
4
Year of publication
1997
Pages
433 - 438
Database
ISI
SICI code
0282-0080(1997)14:4<433:SOTDFR>2.0.ZU;2-5
Abstract
Two azidohexasaccharide building blocks, of which the glycosyl accepto r was the 5-(methoxycarbonyl)pentyl glycoside, were coupled using the trichloroacetimidate technology. The 12 azido functions present in the dodecasaccharide thus formed were then converted to amino groups usin g hydrogen sulfide as a reducing reagent, Subsequent N-acylation with 4-O-benzyl-L-glycero-tetronic acid, followed by catalytic debenzylatio n yielded the desired spacer-equipped, title dodecasaccharide.