Characterization of the promoter of PRS1 in Saccharomyces cerevisiae identifies three regions potentially involved in control of expression

Authors
Hernando, Y Carter, AT Sickinger, S Schweizer, M
Citation
Y. Hernando et al., Characterization of the promoter of PRS1 in Saccharomyces cerevisiae identifies three regions potentially involved in control of expression, J BACT, 183(2), 2001, pp. 795-799
Citations number
20
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
183
Issue
2
Year of publication
2001
Pages
795 - 799
Database
ISI
SICI code
0021-9193(200101)183:2<795:COTPOP>2.0.ZU;2-#
Abstract
The transcription initiation site of the Saccharomyces cerevisiae PRS1 gene was mapped at -179 bp. Measurement of beta -galactosidase activity of the successively deleted PRS1 promoter linked to lacZ and integrated at the ura 3 locus defined three DNA regions involved in the control of PRS1 expressio n. Gel shift analysis confirmed the data.